Відео

who's this Baris guy xd?

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Barış hocam yollarsahoop buradayız

Thank you for this video

hop barıs hocam

Question: what is the purpose of the six "control samples" in the second well if we're using the first well as our standard/control?

Hi! Did I get it right that Stain-free blotting (in order to not make Ponceau) can be performed ONLY on Gels and not on membranes on the "ChemiDoc Touch System", while I can blot gels and membranes on "ChemiDoc MP"?

BARIŞ HOCAM ❤✋

barış hocadan geldikk

So helpful!! Thank you

can i load one sigle set of plates contating gel and no dummy plate on the other side. Will it cause any problem in gel run

thank you for video - but beware - please do not simulate living creatures inside VR simulations... this worm will never be able to send SOS or I am being tortured by pieces of shit which simulate me... its only way it can do that is through gestures of suffocation of fish when caught in a boat, or an ant reacting to pain... these microorganisms feel pain, and if you simulate them, you are provoking a future war beyond your comprehension...

Absolutely the best song ever!!! You guys are seriously amazing! Thank you from a retired bio teacher!

I'm coming from Barış hoca youtube chanel❤

barış hocamdan geldikkk

Came from "DR. BİYOLOJİ" chanel 🤙

Adamlar diyecekler "who is this baris hoca???"

mos

Mr k

Wuhan needs to watch this video 😂

Barıs hoca kalitesiyle buradayız

Durdarsan ki yaad aa gyi

great vid !!!!!!!!

Og song🥰🫶

❤❤❤❤❤❤

this is still my favorite of y'alls!

how does a dead cell that underwent apoptosis appear under the microscope? How to differentiate them from the healthy cells?

You can make a science of everything. Truth is, if you ain't looking for peak performance but just "get that plasmid into that damn cell", it'll be much easier. No need to make your productive lab life harder than it already is with overly correct academic BS, things that are done because they always have been done that way. and might yield 90% efficiency instead of 40%. The beauty of biology is, it can adapt and you as the experimenter usually don't fool around with DNA or cell amounts that leave it up to chance, there is plenty of biological redundancy at work to achieve your goal. Scrap the ice, you don't need it. You got your competent cells, just add your plasmid. Leave them sitting around thawed for a few minutes, then heatshock 30-60s at 42°C. Then put them into growth medium, ideally SOC, maybe LB, anything that nurtures them even if it might be some random soup broth. Let them have their way at ideal temperature for one or two replication cycles (Ecoli 30-40 minutes), then put them into the selective environment by streaking out or liquid inocculation. Longer incubation times will result in redundancy as transformed cells will proliferate, many colonies of the same type, makes it harder to plate select for the correct clone. Not a problem if you put them directly into liquid culture (of course no clone selectivity there, just faith). You could do that in your kitchen, when working with antibiotic resistances as usual. You don't even have to work in a sterile environment, just clean with sterile tools, ignore the laminar flow bench or the benchtop burner for your own convenience. If under these conditions you get contamination on your plates, you got bigger problems than that.

Hello guys .

Barış hocam 🤟🤟

1. siapkan sub sel mini 2. sejajarkan gel sehingga sumur paling dekat dengan elektrode negatif 3. tempatkan egl agarosa ke ruang gel 4. tambahkan buffer elektroforesis ke reservoir sampai reservoir tertutup buffer elektroforesis sedalam 2mm 5. tempatkan sampel sesuai urutan yang benar 6. tempatkan sampel ke dalam sumur dengan menggunakan mikropipet. jagalah pipet tetep tegak lurus terhadap lubang 7. pasang tutup ruang gel sesuai dengan elektrodanya (hitam ke hitam, merah ke merah) 8. sambungkan elektrode ke catu daya 9. nyalakan catu daya 10. atur tegangan konstan sebesar 100V 11. atur timer menjadi 60s 12. amati perubahan yang terjadi

Who else in here for biotech

Thank you so much!

Dr Biyoloji🫡

Interesting that this process has not changed much over the years. I did a high school internship at the Kennedy Krieger Institute back in the late 90's working with X-ALD with mice as our catalyst. I remember being nervous using ethidium bromide after my lab director explained that exposure to it could cause my DNA to mutate!

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What about psi differences ? Im stuck with this troubleshooting Please help

Thankyou 😊

@DR.BİYOLOJİ HOCAMDAN SELAMLAR

😭thank you so much.

Bio-Rad came to my campus' career fair today so I came to listen to this again

SILENCE #yousaidit #haahhahah #wavy #furthersilence #yeahboi

Great video although you could've shown what the samples looked like after all of those steps.

How long should a pH reading remain stable for you to consider it as the recorded pH value?

4:45 just so I understand this... after a 15-minute incubation, the fc block shouldn't wash off the cells, assuming it is able to adhere? Right?

What are the other counter stains other than safranin can be used ?

Anyone for bsc 3rd sem or 4th or 12th

Barış Hocadan gelen arkadaşım sana da selam olsun

Full prepared before 1 hours exam❤😁

Not gonna lie, the organization of this video is annoying. You constantly have to work backwards with some of the information. They don't tell you to sterilize the loop with the first Transfer process, only subsequent transfer processes, same with allow the loop to cool. I am also left guessing whether to sterilize the mouth of the stab culture. I strongly feel it is important to be clear when you are working with bacteria samples and sterilization processes, but I guess this video is better than nothing